Biosynthesis and Characterisation of Ellagic Acid Coupled Silver Nanoparticles- An In-vitro Study
Published: November 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/52213.15599
A Shafie Ahamed, M Chittrarasu
1. Professor and Head, Department of Conservative Dentistry and Endodontics, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram,
Tamil Nadu, India.
2. Associate Professor, Department of Conservative Dentistry and Endodontics, Vivekanandha Dental College for Women, Elayampalayam, Tiruchengode, Namakkal,
Tamil Nadu, India.
Correspondence
M Chittrarasu,
Associate Professor, Department of Conservative Dentistry and Endodontics,
Vivekanandha Dental College for Women, Elayampalayam, Tiruchengode,
Namakkal, Tamil Nadu, India.
Email Id: dr.chittrarasu@gmail.com
Introduction: Nanomedicine dominated the world of therapeutics and diagnostics. Horizons are extending daily to identify a piece of the puzzle that helps us produce eco-friendly and costeffective nanoparticles. In this, authors tried to synthesise a novel Silver Nanoparticle Coated with Ellagic Acid (EA-AgNPs) obtained from pomegranate using a simple method and explore its characterisation precisely.
Aim: To biosynthesise and assess the characterisation of Ellagic Acid coupled Silver Nanoparticles.
Materials and Methods: The present in-vitro study assessed the portrayal of EA-AgNPs using various analytical techniques. Firstly, to analyse: the size of the EA-AgNPs using Ultraviolet (UV)- Visible Spectrometer; Secondly to determine the hydrodynamic size and its dispersity using Dynamic Light Scattering (DLS) and Zeta potential; thirdly to quantify the average size of EA-AgNPs by using Scanning Electron microscope (SEM); and lastly to identify the functional groups by using Fourier Transform Infrared Spectroscopy (FTIR) method. Descriptive statistics were used to analyse the results.
Results: Brownish colour change and shift of peak wavelength from 430-423 nm using UV-Visible Spectrometer analysis confirmed the formation and stability of EA-AgNPs. The DLS analysis revealed that EA-AgNPs were in nanosize (129.7 nm) with less aggregated polydispersity index (0.483). The Zeta potential confirmed that this newly synthesised nanoparticle was negatively charged -0.268 mv. The SEM determination confirmed the formation of spherical-shaped nanoparticles with sizes ranging from 84.34- 98.80 nm. The FTIR revealed EA-AgNPs exhibited different functional groups, which help to prevent particle aggregation.
Conclusion: In this work, the novel EA-AgNPs exhibited the apt characterisation needed for an effective and cost-efficient nanoparticle that could be effectively tapped in various fields of nanodentistry.
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